iroa workflow  (IROA Technologies LLC)

Journal: Nature communications

Article Title: Ion suppression correction and normalization for non-targeted metabolomics.

doi: 10.1038/s41467-025-56646-8

Figure Lengend Snippet: Fig. 8 | Application of IROA-IS workflow in studying mechanisms of L-aspar- aginase (ASNase) resistance through peptide metabolism in ovarian cancer. a IROA-IS Workflows for demonstration of ovarian cancer cell metabolism with or without ASNase treatment. Heatmap demonstrate most altered metabolite

Article Snippet: Ion suppression calculation and corrections Experimental samplesa b c Analytical samples with IS (20μg 13C-IS/Sample) + d IROA-LTRS (20μg 5% U-13C-IS 12C + 20μg 95% U-13C-IS per Sample) a ́ b ́ IROA-IS c ́ d ́ + Blank R el at iv e in te ns ity (% ) 13C monoisotopic 175.1190 M+1 176.1223 Natural abundance carbon is 1.1% 13C Molecule shown = Arginine R el at iv e in te ns ity (% ) m/z m/z Isotopic abundance =~95% IROA 13C 181.1396 180.1363 M-1 Molecule shown = Arginine 12C = 5% 13C = 95% + 175.1190 R el at iv e in te ns ity (% ) IROA 13C (95%)IROA 12C (5%) # of carbons 175.1190 181.1396 12C(5%) 13C(95%) 12C(95%) 13C(5%) R el at iv e in te ns ity (% ) m/zm/z IROA 13C (95%) e IROA-IS IROA-LTRS Isotopic Ratio Outlier Analysis (IROA) protocols d2 ́ m/z In te ns ity Fig. 2 | IROA TruQuant workflow.

Techniques:

Journal: Nature communications

Article Title: Ion suppression correction and normalization for non-targeted metabolomics.

doi: 10.1038/s41467-025-56646-8

Figure Lengend Snippet: Fig. 6 | Dual MSTUS normalization standardizes metabolomic data across a broad range of analytical conditions. Raw MSTUS-12C values (blue lines), nor- malized MSTUS-13C values (green lines), and suppression-corrected MSTUS-12C values (red lines) for indicated chromatographic systems and conditions in plasma. a, b HILIC positive mode, uncleaned and cleaned source; c, d HILIC negative mode, uncleaned and cleaned source; e, f RPLC (C18) positive mode, uncleaned and cleaned source; g, h RPLC (C18) negative mode, uncleaned and cleaned source;

Article Snippet: We present an IROA-based Dual MSTUS Workflow that is applicable across chromatographic systems, ion sources, and biological matrices.

Techniques: Clinical Proteomics, Hydrophilic Interaction Liquid Chromatography

Journal: Nature communications

Article Title: Ion suppression correction and normalization for non-targeted metabolomics.

doi: 10.1038/s41467-025-56646-8

Figure Lengend Snippet: Fig. 8 | Application of IROA-IS workflow in studying mechanisms of L-aspar- aginase (ASNase) resistance through peptide metabolism in ovarian cancer. a IROA-IS Workflows for demonstration of ovarian cancer cell metabolism with or without ASNase treatment. Heatmap demonstrate most altered metabolite

Article Snippet: We present an IROA-based Dual MSTUS Workflow that is applicable across chromatographic systems, ion sources, and biological matrices.

Techniques: